Coding

Part:BBa_K2352015:Design

Designed by: Hannah Schmidt   Group: iGEM17_UFlorida   (2017-10-25)


ARO9(aromatic amino acid aminotransferase II)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 950
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 93


Design Notes

The sequence was optimized for expression in E. coli and made iGEM RFC10 compatible.


Source

The source of the sequence for this part was the Saccharomyces genome database. The sequence was then optimized for E. coli and made RFC10 compatible before being sequenced by IDT.

References